Simple 1X PBS Buffer Recipe For Lab Work

Are you ready to create a perfectly balanced PBS buffer? This 1x PBS buffer recipe is an essential solution for many biological and biochemical applications, including cell culture and molecular biology experiments. Let’s dive into the ingredients and step-by-step instructions that will help you whip up this versatile buffer solution!

Image of PBS Buffer Recipe

First, take a look at this helpful image that outlines the PBS buffer recipe. It will guide you through what you need for this essential preparation.

Ingredients for 1x PBS Buffer

Gather the following ingredients to successfully create your 1x PBS buffer:

8 g of sodium chloride (NaCl)
0.2 g of potassium chloride (KCl)
1.44 g of disodium phosphate (Na2HPO4)
0.24 g of monosodium phosphate (NaH2PO4)
Up to 1 L of distilled water

Instructions to Prepare 1x PBS Buffer

Follow these simple instructions to prepare your 1x PBS buffer solution:

Begin by measuring the specified amounts of each ingredient with precision.
In a suitable container, combine the sodium chloride, potassium chloride, disodium phosphate, and monosodium phosphate. Ensure all solids are thoroughly mixed.
Add approximately 900 mL of distilled water to the container with the powdered ingredients. Stir the mixture gently until all solids have completely dissolved.
Once the solid ingredients are dissolved, add additional distilled water to bring the total volume of the solution up to 1 liter.
Finally, adjust the pH of the buffer to approximately 7.4 using hydrochloric acid (HCl) or sodium hydroxide (NaOH) as needed. Monitor the pH with a pH meter or litmus strips to ensure accuracy.
Your 1x PBS buffer is now ready for use. Store any unused buffer in a clean, labeled container at room temperature or refrigerated for future experiments.

Having this 1x PBS buffer recipe at your fingertips is invaluable for a plethora of lab procedures, from washing cells to diluting samples for assays. Enjoy your time in the lab, and happy experimenting!